Christian Dubos PhD Student



 

Date of birth : 21/01/1974
 

Citizenship: French

INRA - Equipe de Génétique et Amélioration des Arbres Forestiers

B.P. 45
33610 CESTAS Cedex
FRANCE
Dubos@pierroton.inra.fr

 
 
Last modified on 28th august 2001

Keywords

Transcriptomics – cDNA-AFLP – SSH - Library – Proteomics – 2D-PAGE – Genomics – Plant Genetics – Forest tree genetics – Drought Stress – Molecular Genetics – Molecular markers – Genetic mapping – QTL – PQL –  Marker Assisted Selection – Macro-arrays

Academic Qualifications

        _ "DEUG" in Nature and Life Sciences (1993-1995, Université Paul Sabatier, Toulouse, France)

        _ "Licence - Maîtrise" in  Plant Cellular Biology and Physiology  (1994-1996,Université Paul Sabatier, Toulouse, France)

        _ "DESU" experimental work done in the laboratory of "Expression et Modulation de la Lignification" under the
          responsability of Grima-Pettenati J and Boudet AM (1995, Université Paul Sabatier - CNRS, Toulouse, France)
          Subject:
          Tools development for molecular evolution studies: case of cinnamyl alcohol dehydrogenase and cinnamoyl CoA
          reductase (Directed PCR, Cloning, Sequencing, Dendrogram)

       _ Master in Forestry Biology ( 1997-1998, Ecole Doctorale de Nancy, France), experimental work done in the
          laboratory of "Génétique et Amélioration des Arbres Forestiers" (INRA, Bordeaux-Pierroton, France) under the
          responsability of Christophe Plomion
          Subject:
          Genetic studies of maritime pine proteins (2D-PAGE, Mapping, QTL and PQL analysis)

        _ PhD Thesis in Forestry Biology (1998-2001, Ecole Doctorale de Nancy, France), experimental work done in the
           laboratory of "Génétique et Amélioration des Arbres Forestiers" (INRA, Bordeaux-Pierroton, France) under the
           responsability of Christophe Plomion
           Subject:
           Maritime pine transcriptome reaction to an hydric stress (cDNA-AFLP, Cloning, Sequencing, Mapping, QTL analysis,
           Macro-arrays)

Technical Experience

Projects in which I am implicated

National projects:
 
JM Guehl, C Plomion (07/99-07/01) Déterminants génétiques et moléculaires de l’efficience d’utilisation de l’eau et de la tolérance à la sécheresse chez le pin maritime . ECOGENE (INRA)
 
European projects:
 
FORADAPT (INCO-DC, ERBIC 18CT 970 200, DG XII, coordinator). Global, physiological and molecular responses to climatic stresses of three mediterranean conifers (Pines of the halepensis-brutia section, maritime pine and mediterranean cedars). Consequences for optimal use, conservation and sustainable improvement of their genetic ressources. 01/09/97-31/08/01
 
DELTA (FAIR CT95 – 0781, DGVI FII.3, participant). Genetic, molecular and physiological determinants of water-use efficiency and drought resistance in major forest trees. 03/96-02/99

Publications

    Costa P, Pionneau C, Bauw G, Dubos C, Bahrman N, Kremer A, Frigerio J-M, Plomion C (1999) Separation and characterization of needle and xylem maritime pine proteins. Electrophoresis 20: 1098-1108
     
    Costa P, Pot D, Dubos C, Frigerio J-M, Pionneau C, Bodénès C, Bertocchi E, Cervera M-T, Remington DL, Plomion C (2000) A genetic map of Maritime pine based on AFLP, RAPD and protein markers. Theor Appl Genet 100:39-48

    Dubos C, Plomion (2001) Drought affects expression of a PR-10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlings. J Exp Bot 52: 1143-1144

    Dubos C, Plomion (2001) Identification of water-deficit responsive genes in maritime pine (Pinus pinaster Ait.) roots using non radioactive cDNA-AFLP, and quantitative expression analysis using reverse northern. Plant mol Biol (Submited)

    Dubos C, LeProvost G, Pot D, Salin F, Lalane C, Madur D, Frigerio J-M, Plomion C (2001) Identification and characterization of water-deficit responsive genes in hydroponically grown maritime pine (Pinus pinaster Ait.) seedlings. Plant Physiol (Submited)

   Book chapter

    Costa P, Pionneau C, Bauw G, Dubos C, Bahrman N, Kremer A, Frigerio J-M, Plomion C (2000) Separation and characterization of needle and xylem maritime pine proteins. In : Dunn M.J. (ed.) "From Genome to Proteome", Wiley-VCH, Weinheim, pp518-528

   Poster – Oral presentation

    Costa P, Dubos C, Pionneau C, Pot D, Plomion C (1998) Mapping PQLs (protein quantity loci) for drought responsive proteins in maritime pine. Abstracts of the 6th international conference of the Plant and Animal Genome, San Diego, CA, January 14-18. Abstr. # 287 (Poster)
     
    Costa P, Morteau A, Dubos C, Pionneau C, Pot D, Queyrens A, Guye M, Guehl J-M, Frigerio J-M, Kremer A and Plomion C (1998) Physiological and molecular response of Maritime pine to progressive water deficit. Abstracts of the 6th international conference of the Plant and Animal Genome, San Diego, CA, January 14-18. Abstr. # 47 (Oral presentation)
     
    Costa P, Dubos C, Kremer A, Plomion C (1998) Quantitative analysis of maritime pine protein expression in two contrasted environments. « From Genome to Proteome », 3rd Siena 2D Electrophoresis meeting, 31 august - 3 september (Poster)
     
    Plomion C, Costa P, Pionneau C, Bauw G, Dubos C, Bahrman N, Kremer A, Frigerio J-M (1998) Separation and characterization of needle and xylem maritime pine proteins. In : « From Genome to Proteome », 3rd Siena 2D Electrophoresis meeting, 31 august- 3 september. (Oral presentation)
     
    Plomion C, Costa P, Dubos C, Frigerio J-M, Guehl J-M, Guy M, Kremer A, Lambrot C, Loustau D, Morteau A, Pastuszka P, Pionneau C, Pot D, Queyrens A, Raffin A, Roussel G, Sartore M (1999) An integrative study of drought stress response in maritime pine. Joint meeting DELTA/MPC/FORADAPT, Orvieto (Italie) 05/30-06/1 (Oral presentation)
     
    Plomion C, Costa P, Dubos C, Frigerio JM, Pionneau C (1999) Genetics of the maritime pine proteome. Forest Biotechnology '99, Oxford July 11-16 (Oral presentation)
     
    Dubos C, Frigerio J-M, Lalanne C, Madur D, Plomion C (1999) Identification of drought-stress regulated genes in maritime pine. Forest Biotechnology '99, Oxford July 11-16 (Poster)
     
    Plomion C, Costa P, Dubos C, Frigerio J-M, Guehl J-M, Queyrens A (1999) Genetical, physiological and molecular response of Pinus pinaster to a progressive drougth stress. European Science Fondation, Scientific programme on Plant Adaptation "Adaptation to abiotic stress: tolerance and avoidance", Kevo, 15-19 Sept 1999, Finlande (Oral presentation)
     
    Plomion C, Costa P, Dubos C, Frigerio J-M, Queyrens A, Guehl J-M, Kremer A (1999) Quantitative analysis of drought stress response at the physiological and molecular levels. IUFRO Conference (Division 2 and 7). Genetic response of forest systems to changing environmental conditions, Analysis and management. Freising , Sept 12-17, Germany (Poster)

    Plomion C, Costa P, Dubos C, Frigerio J-M, Queyrens A, Guehl J-M, Kremer A (1999) Quantitative analysis of drought stress response at the physiological and molecular levels. IUFRO Conference (Division 2 and 7). Genetic response of forest systems to changing environmental conditions, Analysis and management. Freising , Sept 12-17, Germany (Oral presentation)

    Plomion C, Costa P, Dubos C, Frigerio JM, Pionneau C (2000) Proteome analysis in maritime pine. Abstracts of the 8th international conference of the Plant and Animal Genome, San Diego, CA, January 9-13. Abstr. # W109 (Oral presentation)

    Plomion C, Costa P, Dubos C, Frigerio J-M, Queyrens A, Guehl J-M (2000) An integrative study of drought stress response in maritime pine (Pinus pinaster). Abstracts of the 8th international conference of the Plant and Animal Genome, San Diego, CA, January 9-13. Abstr. # W5 (Oral presentation)

    Frigerio J-M, Dubos C, Plomion C (2000) Gene expression in shoots and roots of well watered and drought-stressed maritime pine seedlings. Abstracts of the 8th international conference of the Plant and Animal Genome, San Diego, CA, January 9-13. Abstr. # 590 (Poster)

    Dubos C, Plomion C (2000) Identification and characterisation of maritime pine cDNAs involved in drought stress response. New frontiers in Plant Science and Plant Biotechnology, Toulouse, France, March 5-8, Session I, Plant Genome and Genomics (Poster).

    Plomion C, Costa P, Dubos C, Frigerio J-M, Queyrens A, Guehl J-M, Kremer A (2000) Quantitative analysis of drought stress response at the physiological and molecular levels. New frontiers in Plant Science and Plant Biotechnology, Toulouse, France, March 5-8, Session I, Plant Genome and Genomics (Poster).

    Dubos C, Plmion C (2001) Molecular genetics of drought stress in maritime pine. Abstracts of the 9th international conference of the Plant and Animal Genome, San Diego, CA, January 13-17. Abstr. # W2 (Oral presentation).

    Frigerio J-M, Dubos C, Plomion C (2001) Digital northern in maritime pine seedlings faced to drought. Abstracts of the 9th international conference of the Plant and Animal Genome, San Diego, CA, January 13-17. Abstr. # P61 (Poster).

    Chagne D, Scotti I, Jansson G, Madur D, Lalanne C, Frigerio J-M, Brown G, Neale D, Dubos C (2001) Comparative mapping in conifers. Abstracts of the 9th international conference of the Plant and Animal Genome, San Diego, CA, January 13-17. Abstr. # P553 (Poster).

    Plomion C, Le Provost G, Pot D, Dubos C, Frigerio J-M, Chantre G, Rozenberg P, Pichavant F (2001) Genetic and molecular determinism of wood quality in maritime pine.  Abstracts of the 9th international conference of the Plant and Animal Genome, San Diego, CA, January 13-17. Abstr. # P554 (Poster).

    Dubos C, Krier C, Frigerio J-M, Le Provost G, Lalanne C, Madur D, Pot D, Brendel O, Plomion C (2001) Molecular genetics of drought stress response in maritime pine. Abstracts of the WBB conference. International conference on: Wood, Breeding, Biotechnology and Industrial expectations. Bordeaux, France, June 11-14, Session 7. (Oral presentation).

    Le Provost G, Canovas F, Avila C, Canton FR, Herrera R, Mouret JF, Paiva J, Brach J, Dubos C, Plomion C (2001) Identification of genes differentially expressed in xylem associated with different types of wood in maritime pine. Abstracts of the WBB conference. International conference on: Wood, Breeding, Biotechnology and Industrial expectations. Bordeaux, France, June 11-14, Session 7. (Oral presentation).

    Plomion C, Cahalan C, Canova F, Chagne D, Chantre G, Dubos C, Fevereiro P, Frigerio J-M, Grabner M, Hofte H, Lelu M-A, Le Provost G, Mouret J-F, Pereira H, Pichavant F, Pot D, Rozenberg P (2001) Genetic and molecular determinism of wood quality in maritime pine. Abstracts of the WBB conference. International conference on: Wood, Breeding, Biotechnology and Industrial expectations. Bordeaux, France, June 11-14, Session 1, Abstr.# 1. (Poster).