I - RESEARCH ACTIVITIES IN POPULATION AND EVOLUTIONARY GENETICS

I. 1. Population and evolutionary genetics in European temperate oaks

- Cytoplasmic DNA polymorphism in oaks

- Molecular organisation of species differentiation in closely related oak species

- Inter and intraspecific gene flow and paternity analyses in mixed oak stands using microsatellites

I. 2. Population genetics of tropical trees

- Levels of diversity of tropical trees with contrasting life history traits

- Mating systems and sexual reproduction in tropical trees

- Spatial distribution of cpDNA polymorphism in tropical trees

I.3. Population genetics in maritime pine

I.4. Methodology in population genetics

- Estimation of diversity and differentiation measures

- Simulation of postglacial recolonization

- Simulation of spatial genetic structures

II - RECENT RESEARCH ACCOMPLISHMENTS IN GENETIC MAPPING AND TRAIT DISSECTION STUDIES

II.1. Construction of genetic maps in Maritime pine, Eucalyptus, Oak and Norway spruce

- Mapping in Maritime pine : Linkage maps

- Mapping in Eucalyptus : Linkage maps

- Mapping in Oak : Linkage maps

- Mapping in Norway spruce : Linkage maps

II.2. QTL analysis of commercial traits - Marker-assisted selection

II.3. Genetic dissection of complex traits

III - PROTEOME ANALYSIS

III.1. Two dimensionnal gel electrophoresis of proteins

III.2. Genetic mapping of protein markers

III.3 Water stress responsive proteins

III.4 Proteins involved in wood formation

IV - GENOME ANALYSIS

V - BREEDING AND TREE IMPROVEMENT

VI - LABORATORY PROTOCOLS

1 - Proteins extraction, 2D electrophoresis and silver staining

2 - DNA extraction

3 - RAPD

4 - CpDNA and plant mtDNA primers

5 - PCR - RFLP

6 - SSCP

7 - Microsatellites

8 - AFLP

VII - Molecular marker for linkage analysis in forest trees: literature review

VIII - Summaries of our recent publications

STAFF

Home pages :

• Christain Dubos
• Caroline Saintagne