Primer pairs used as comparative anchor tags for comparative mapping in conifers
link to the Conifer Comparative Genomics Project (USDA)
last update August 25, 2000
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Amplification conditions |
Linkage groupg |
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|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
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Gene (accession) ((species)) |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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4CL |
U: CCCCGTCAAATCTGGCTCCT |
1127 |
1400 |
68 |
2.5 |
1600+500 |
48-56 |
2 |
1400 |
68 |
2.5 |
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(U310404) |
L: GGGCGCTTACTCTGCACCAC |
genomic clone |
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(60-68) |
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52 |
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(60-68) |
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((Pinus taeda)) |
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Amplification conditions |
Linkage groupg |
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|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
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|
Gene (accession) ((species)) |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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COMT-1 |
U: AACGCCAGAATTGTGATGAA |
453 |
870 |
64 |
3.0 |
600 |
60 |
1.5 |
1000 |
61 |
1 |
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(U310301) |
L: GGAACTATGCGCCACCATTA |
cDNA clone |
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((Pinus taeda)) |
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Amplification conditions |
Linkage groupg |
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|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
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|
Gene (accession) ((species)) |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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PAL |
U: TAGCCAAGAAAACCCTGAG |
452 |
452 |
52TD |
2.5 |
450 |
48-58 |
2 |
452 |
54 |
2 |
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(U3107102) |
L: ACTGATAGCGTCGTAAACCA |
genomic clone |
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56 |
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((Pinus taeda)) |
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Amplification conditions |
Linkage groupg |
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|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene (accession) ((species)) |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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CAD |
U: GTCCCCTTACACTTACAATC |
156 |
760 |
55 |
2.0 |
450 |
52 |
2 |
NA |
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(Z371010) |
L: ATCCCCACCACTTCAT |
cDNA clone |
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((Pinus taeda)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
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|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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ARA554 |
U: CAGCAATGACGATGGTTTTA |
306 |
306 |
52 |
1.5 |
MBP |
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306 |
52 |
1.5 |
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(U010554) |
L: GGCTGGGGACGAGAAC |
cDNA clone |
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(50-56) |
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(Multi Banding |
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(50-56) |
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((Pinus taeda)) |
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Pattern) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
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|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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ARA556 |
U: GAAACCCGGGGCTCTGAACT |
455 |
455 |
56 |
1.5 |
NA |
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455 |
56 |
1.5 |
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(U010556) |
L: CCGCAGCGAGGAAAACAA |
cDNA clone |
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(56-64) |
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(No Amplification) |
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(56-64) |
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((Pinus taeda)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
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|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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CHS |
U: ACTCCCCCTAATGCGGTTGA |
370 |
370 |
64 |
2.0 |
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370 |
64 |
2.0 |
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(X60754) |
L: CTTGGCTGCGGCTTCTTTC |
genomic clone |
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(54-64) |
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(54-64) |
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((Pinus sylvestris)) |
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|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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STS |
U: CTGTAGGAATGGGCGTTGAT |
776 |
NA |
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NA |
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( X60753) |
L: GATTGCTCCGTCGCTGTT |
cDNA clone |
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((Pinus sylvestris)) |
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|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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ICL |
U: GGGGTAACACTGGCAATGTT |
678 |
1600 |
56 |
2.0 |
NA |
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1600 |
56 |
2.0 |
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(U310807) |
L: GCACAGTACCCGCACCAA |
cDNA clone |
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(54-60) |
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(54-60) |
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((Pinus taeda)) |
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|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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PCHILDE |
U: CTGGGCCACTTTCTTCTATC |
1047 |
1000+1020 |
60 |
1.5 |
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1047 |
60 |
1.5 |
unlinked |
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(X66727) |
L: CAAGCCCTCTCAGGTCAC |
genomic clone |
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((Pinus taeda)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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NIR |
U: AAAGCAAGAGCCCTGAAAAT |
303 |
303 |
56 |
2.0 |
NA |
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303 |
56 |
2 |
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(X7410410) |
L: CCTGATGGCTCCAAAGTG |
cDNA clone |
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(54-60) |
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(50-60) |
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((Pinus sylvestris)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
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|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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SODchl |
U: TCCGTTTGACAGGATTGACT |
284 |
1050 |
60 |
2.0 |
1000 |
54 |
2 |
1050 |
60 |
2.0 |
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( X585710) |
L: CCCCAGGTCATCCTCTAACT |
cDNA clone |
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(50-62) |
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(50-62) |
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((Pinus sylvestris)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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SODcyt |
U: TGGGTCTTCTTAAGGCTGTT |
383 |
700 |
48 |
2.5 |
1000 |
48-54 |
2.5 |
NA |
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( X58578) |
L: GCCAAGATCATCAGGATCAG |
cDNA clone |
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(48-50) |
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((Pinus sylvestris)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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GS |
U: AAGTTGGGCTGCACGT |
600 |
1300 |
58 |
1.5 |
NA |
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1300 |
58 |
1.5 |
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unpublished |
L: TTGGAAGCTGGCCTGC |
cDNA clone |
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52-60 |
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52-60 |
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((Pinus sylvestris)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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GLP |
U: GGGAGGCCATGTCTGAAT |
2010 |
2010 |
52 |
2.0 |
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2010 |
52 |
2.0 |
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(AF0310201) |
L: GGGTGAATATGGGGAGGTA |
cDNA clone |
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(48-56) |
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(48-56) |
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((Pinus caribaea)) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
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lp2 SAM |
U: GATTTGCCGTGCTGTTTGCT |
631 |
1400 |
58 |
1.5 |
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1400 |
58 |
1.5 |
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synthase |
L: TCGTGCTGAGTGGAGATGAGAA |
cDNA clone |
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(58-66) |
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(58-66) |
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((Pinus taeda)) |
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|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
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|
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lp3-1 ABA/ripening inducible protein |
U: GGAGGAGAAACAGCACCAC |
716 |
MBP |
48-58 |
2.5 |
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|
716 |
62 |
2 |
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(U52865) |
L: CGGAAATCACACGAAAAGAA |
cDNA clone |
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((Pinus taeda)) PtIFG28810 |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
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|
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lp4 stellacyanin |
U: ACATACACCAGTGGCAACGA |
5810 |
1000 |
52 |
2.0 |
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NA |
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((Pinus taeda)) |
L: GACGGACATGAACCCAATG |
cDNA clone |
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(50-52) |
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Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
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|
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lp5 glycin rich protein |
U: CCGGACTTCTGTTTGCTT |
773 |
MBP |
63 |
2.0 |
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|
smear |
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(AF013805) |
L: ATGGCCACACAATACACTGT |
cDNA clone |
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((Pinus taeda)) |
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|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
lp6 chitinase homolog |
U: TTTCGCTTCCCTTCATTTTC |
787 |
MBP |
56 |
2.0 |
|
|
|
NA |
|
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|
(U313010) |
L: TTCCACATTCGGCATCTG |
cDNA clone |
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((Pinus taeda)) |
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|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
lp8 Ca Binding protein |
U: TGATTTCGGAGGCGGAGAT |
461 |
461 |
64 |
1.5 |
MBP |
|
|
461 |
64 |
1.5 |
|
|
|
|
(AF013802) |
L: GCGCTGAGAGCTGAGGTAGG |
cDNA clone |
|
(50-64) |
|
|
|
|
|
(50-64) |
|
|
|
|
|
((Pinus taeda)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
ptxmyb413 (myb I) |
U: TCACCCCAGGAAGAACAAT |
2610 |
660 |
56 |
1.0 |
MBP |
|
|
540 |
56 |
1.5 |
|
|
|
|
(unpublished) |
L: AATGAGGTTGTTGGTGGC |
genomic clone |
|
(51-56) |
|
|
|
|
|
(48-58) |
|
|
|
|
|
((Pinus taeda)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
ptxmyb126 (mybII) |
U: AGTGGGATTGAAGAAGGGT |
500 |
450 |
52 |
2.0 |
280 |
50 |
1.5 |
450 |
52 |
1.5 |
|
|
|
|
(unpublished) |
L: CCAACGCAATCTACAACT |
genomic clone |
|
(51-54) |
|
|
|
|
|
(51-54) |
|
|
|
|
|
((Pinus taeda)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
Peroxydase (compression |
U: CCGATGCTGCTCTTCTAAC |
475 |
500 |
52 |
2.0 |
MBP |
|
|
|
|
|
|
|
|
|
wood responsive) |
L: AGCGAATTTGGAGGATGA |
cDNA |
|
|
|
|
|
|
|
|
|
|
|
|
|
(AF028073) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
((Pinus taeda)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
Ferritine ((compression |
U: ATGGAGCTGACTTTGTCTTT |
4510 |
800 |
57 |
2.5 |
550 |
48 |
3 |
|
|
|
|
|
|
|
wood responsive) |
L: GAGGGCTAATTGAGAAACAG |
cDNA |
|
|
|
1000 |
|
|
|
|
|
|
|
|
|
(AF028072) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
((Pinus taeda)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
Myrcene Synthase |
U: GGCTTGGGAGGCTTATTTTG |
cDNA |
NA |
|
|
300 |
50-52 |
2 |
|
|
|
|
|
|
|
(U871008) |
L: GGGCATCTTCTTCGGTTGA |
335 |
|
|
|
|
|
|
|
|
|
|
|
|
|
((Abies grandis)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
Pinene synthase |
U: CCTCCCTCATTGCCTTTCC |
cDNA |
800+600 |
60 |
2.0 |
MBP |
|
|
|
|
|
|
||
|
(U8710010) |
L: AACCCGATTCTTTCCACCAT |
327 |
|
|
|
|
|
|
|
|
|
|
|
|
|
((Abies grandis)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
Limonene synthase |
U: GGCTCTCCTTTCTATCGTAT |
cDNA |
350 |
52-57 |
2.0 |
MBP |
|
|
|
|
|
|
|
|
|
(AF0061103) |
L: CCACCACCATTATCATCA |
1105 |
|
|
|
|
|
|
|
|
|
|
|
|
|
((Abies grandis)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
SAM lp2 drought inducible |
U: CCGACAGCAAGGTAGCAT |
cDNA |
380 |
56-54 |
2.0 |
350+500 |
60 |
2 |
|
|
|
|
|
|
|
(U38186) |
L: GGGGCAGGTTCCATTCT |
380 |
|
|
|
|
|
|
|
|
|
|
|
|
|
((Pinus banksiana)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
|
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
RbcS Ribulose Biphosphate Carboxylase Small Subunit |
U: ACC CCA AGT TTG AGA CTC TG |
331 |
450 |
56-58 |
2 |
450 |
56-58 |
2 |
|
|
|
|
|
|
|
(X13408) |
L: GGC TTG TGG ACG ATG AAG |
cDNA |
|
58 |
|
|
58 |
|
|
|
|
|
|
|
|
((Pinus thunbergiana)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from conifer genes available in GenBank (a) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene (accession) ((species)) |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
Pepc : Phosphoenolpyruvate carboxylase |
U: GCT GCC TTC CGC ACA GAT GAA A |
1150 |
|
|
|
|
|
|
|
|
|
|
|
|
|
(X7100100) |
L: CCT ACC AGC ATC CTT TCC AGA A |
cDNA |
1800 |
48 |
3 |
1800 |
48 |
3 |
|
|
|
|
|
|
|
((Picea abies)) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG11017 |
U: ATATCCGTCGCCTGGTTAAG |
320 |
NA |
|
|
500 |
58 |
2 |
|
|
|
|
|
|
|
|
L: GATTCTCAAAGCAGCCCAAG |
cDNA |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG1584 deoxychalcone synthase/NADPH reductase |
U: CGAAGCAAAGGATGTCACG |
|
340 |
(54-56) |
1.5 |
350 |
56-58 |
2 |
|
|
|
|
|
|
|
|
L: TGTTGAGGTGGGGATTGG |
cDNA |
|
54 |
|
|
56 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG1750 |
U: TGTTTACGTTCTTGACGCGG |
300 |
340 |
54 |
2.0 |
NA |
|
|
|
|
|
|
|
11 |
|
|
L: TAGCAAGCACTCTGACTGTGG |
cDNA |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG1165 |
U: CTGAGGGGATGTGCATGAC |
250 |
360 |
(62-60) |
2.0 |
MBP |
|
|
|
|
|
|
|
6 |
|
|
L: AAACCAGCAGCCACATGAG |
cDNA |
|
60 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG7310 |
U: TACAAAGGCTCGGGTATTGG |
350 |
350 |
(56-58) |
2.0 |
400 |
(48-58) |
2 |
|
|
|
6 |
||
|
|
L: GTCATGCCTAACAAGCCCTG |
cDNA |
|
58 |
|
|
56 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG107 Beta chain chloroplast ATP synthase |
U: GCAGGACCTTCTGGACAATC |
450 |
420 |
(60-51) |
1.5 |
MBP |
|
|
|
|
|
|
|
1 |
|
|
L: AGGTGGAGAAAGCCAAGCTC |
cDNA |
|
60 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG22100 light harvesting complex chlorophyll a/b binding |
U: AGCTTGCAGCATCAACCG |
650 |
820 |
(61-641) |
2 |
300 |
50 |
2 |
|
|
|
|
|
10 |
|
|
L: GAACCAAACAGCTTCAGGACC |
cDNA |
|
62 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG11056 |
U: GAAGCTAGCGAAGGCTTTGG |
410 |
350 |
(51-52) |
01/05/2000 |
350+450 |
48-58 |
01/05/2000 |
|
|
|
|
10 |
|
|
|
L: GGGTGTGACCATATAACACCG |
cDNA |
|
51 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG1576 |
U: TGGTATGTGGAGGGAAGGC |
480 |
380 |
(61-60) |
1.5 |
MBP |
|
|
|
|
|
|
|
1 |
|
|
L: TACAGCGTTTGCTCCTCCTG |
cDNA |
|
60 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
|
Picea abies |
|
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG464 plasma membrane intrinsic protein |
U: TGTCACTGCCCAGAGCTATTC |
450 |
600 |
(60-55) |
1.5 |
650 |
50 |
3 |
|
|
|
|
|
|
|
|
L: ATCACAGCCGCTCCAAAAC |
cDNA |
|
58 |
|
1300 |
|
|
|
|
|
|
2 |
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (b) |
Pinus pinaster |
|
Picea abies |
|
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
PtIFG624 |
U: CACAATTGCCAGATGGGTC |
620 |
1000 |
(61-57) |
1.5 |
1000 |
48-58 |
2 |
|
|
|
|
10 |
|
|
protein kinase |
L: CCTCTCTAGCAACGATCCG |
cDNA |
|
60 |
|
|
52 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
606, entE gene |
U: TCA TGA GGG GAG AAA GTT GG |
360 |
|
|
|
|
|
|
|
|
|
6 |
||
|
|
L: GGG CAG TCA CTT GAA CTT CG |
cDNA |
|
|
|
|
|
|
|
|
|
|||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
1643, ABI1 gene product |
U: AAT GGA GGA TGC CGT TAC AG |
4100 |
|
|
|
|
|
|
|
|
|
|
10 |
|
|
(H751101) |
L: AAC CAC TCT CGA ATC CCC AC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
1764 |
U: TGA TCG GTG TTC AGG CAA G |
|
|
|
|
|
|
|
|
|
|
|
6 |
|
|
(H75123) |
L: TGTCTCACCCTCCTCCAAAG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
2274 |
U: TGA TCA GAG AGC TGG TGC AG |
400 |
|
|
|
|
|
|
|
|
|
|
4 |
|
|
(H75066) |
L: AGA TGA GCA TCA GGT CAG CC |
|
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
2358 |
U:GTT AAC CCT CGA GGA GAC ATG |
330 |
|
|
|
|
|
|
|
|
|
|
6 |
|
|
|
L: GCT TCC ACA GTC CAC AAT CTG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
84210 |
U: cgcgcggGTTGAAAGCGACTCCAAAGG |
|
|
|
|
|
|
|
|
|
|
|
4 |
|
|
(AA7310505) |
L: GAGGCATTTATGAGGGAACG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
8436 |
U: cgcgcggGACAAGGATCCAACTCTCAGC |
|
|
|
|
|
|
|
|
|
|
|
7 |
|
|
(AA7310508) |
L: TGAACTTGTCGGTGCTTAGG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
8580 |
U: TGGAAACCGTCTACAGTCGC |
|
200 |
48 |
3 |
|
|
|
|
|
|
|
10 |
|
|
|
L: ACTGGATTCCGGAGGATCAC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
8656 |
U: CACCAAGTACACCACAACACC |
200 |
|
|
|
|
|
|
|
|
|
unlinked |
|
unlinked |
|
(AA7310632) |
L: ATTTGTGGCTGCGGAAAG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
8702, Thioredoxin |
U: AGT CGC ACT TGC TCC AGT TC |
2100 |
|
|
|
|
|
|
|
|
|
|
6 |
|
|
(AA7310652) |
L: GTT GCA GAA AAG GGT GGC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus |
Picea |
Pinus |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
pinaster |
abies |
taeda |
|
87710 |
U: TCAAGTCAGTTGCTTGAACATC |
|
|
|
|
|
|
|
|
|
|
|
5 |
|
|
(AA7310708) |
L: ccggccgggAGGATTCGCGGTGAAAGG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
88108, plasma membrane intrinsic protein WD40-repeat type I |
U: ATG GGG GTG CAG CAT AAA C |
330 |
|
|
|
|
|
|
|
|
|
|
4 |
|
|
|
||||||||||||||
|
(AA73107106) |
L: GGG ATG GCA ACA ACA AAA AG |
cDNA |
|
|
|
|
|
|
|
|
|
|||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
81007, cationic peroxidase |
U: TGG GTG AGA CTT ACG AAC AGG |
300 |
|
|
|
|
|
|
|
|
|
|
8 |
|
|
(AA73107107) |
L: TCC TCA ACA GGC AGA CCT TC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
810310, ribosomal protein 40S S16 |
U: AAC CAC GAG CTT GGC ATG |
300 |
|
|
|
|
|
|
|
|
|
|
2 |
|
|
(AA7310822) |
L: ACG TGG ACG AGC AGT CAA AG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
81072, protein 2 major intrinsic plasma membrane |
U: GCC TCC ATT CGA CTC ACT TG |
310 |
|
|
|
|
|
|
|
|
|
|
6 |
|
|
(AA7310836) |
L: TTG GTC CCC TTG TTG GAG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
10036, ribosomal protein L37 |
U: GTC ATC CGA TAC AAC CTC AAT C |
250 |
|
|
|
|
|
|
|
|
|
|
7 |
|
|
(AA7310871) |
L: CAG GAC GAA TGA GAT ACC TGC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
10044, ribosomal protein 40S S27 |
U: CAT CGC ATC AGT CAT ACT CAC C |
280 |
|
|
|
|
|
|
|
|
|
|
6 |
|
|
(AA7310876) |
L: CGC GGC CCA ACT GGA GGA AAA GCA CGA C |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
100102, nonspecific lipid transfer protein |
U: AGCTAAAGTTGGCTGGCATC |
270 |
|
|
|
|
|
|
|
|
|
|
5 |
|
|
(AA73101007) |
L: GCCGGGTCACTGACCTTAACGTCCC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
10136, ribosomal protein S11 |
U: CCGATTTGAAACACACATCC |
260 |
|
|
|
|
|
|
|
|
|
|
3 |
|
|
(AA73101040) |
L: GCCGGCCGGGCCCTTGTCCAAGACTGTAAG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
10151, Cucumber basic protein, a blue copper protein electron |
U: GCAGAATCTCAGCAGCAATG |
2100 |
|
|
|
|
|
|
|
|
|
|
7 |
|
|
|
||||||||||||||
|
(AA73101054) |
L: TAGTGAGCCCTGGAGCGTAC |
cDNA |
|
|
|
|
|
|
|
|
|
|||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
464, plasma membrane intrinsic protein |
U: TGT CAC TGC CCA GAG CTA TTC |
450 |
|
|
|
|
|
|
|
|
|
|
|
|
|
(H75150) |
L: ATC ACA GCC GCT CCA AAA C |
cDNA |
|
|
|
|
|
|
|
|
|
|
2 |
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
8103, non specific lipid transfer protein |
U: GGA CTG AAG GGA TCT AGC TGG |
450 |
|
|
|
|
|
|
|
|
|
|
5 |
|
|
(H75118) |
L: CAG CCC AAA TTC CAT CGT C |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
1623, Gry-rbp |
U: TTT CTC AGG TGG GAG AGG TG |
350 |
|
|
|
|
|
|
|
|
|
|
2 |
|
|
(H751010) |
L: TCG CAT CCA TGT GCG TAG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
2781 |
U: GAT GAT GCC CTG AAG AGC C |
450 |
|
|
|
|
|
|
|
|
|
|
8 |
|
|
|
L: ATG GAA CCA AAG GAG ATG CC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
48, aquaporin |
U : GAC ATT TGG ACT CTT CCT GGC |
270 |
|
|
|
|
|
|
|
|
|
|
2 |
|
|
(H75016) |
L : AAC ATG AGA ATC ACG GGC AC |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
7310 |
U : TAC AAA GGC TCG GGT ATT GG |
350 |
|
|
|
|
|
|
|
|
|
|
6 |
|
|
(H75167) |
L : GTC ATG CCT AAC AAG CCC TG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
8732 |
U : gccATGCACAGGATGAACCCTTC |
|
|
|
|
|
|
|
|
|
|
|
8 |
|
|
(AA7310680) |
L : ggcccggcggccggTGAAGTTCTGAGTTTGGCGG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus taeda ESTs (c) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
10076, Phosphoglucomutase |
U : CTC TAT TGC AAA AAT GTG CCA C |
250 |
|
|
|
|
|
|
|
|
|
|
11 |
|
|
(AA73108107) |
L : AGAATTTACTGGCCGCTCG |
cDNA |
|
|
|
|
|
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01A05 |
U: TCG TCT CGC ATG GCA CTC TG |
cDNA |
200 |
50-58 |
2 |
MBP |
|
|
|
|
|
|
|
|
|
Cytosolic ascorbate peroxidase |
L: GCA GCC TGA TCG CAA TGT CA |
131 |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01B07 |
U: TGT GAA AAG GAA GCG GTG AA |
cDNA |
250 |
50-58 |
2 |
250+450+800 |
48-56 |
2 |
|
|
|
|
|
|
|
Chloroplastic carbonic anhydrase |
L: GGA AGA GTG ATG GCA AGC AA |
237 |
|
50 |
|
|
50 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01B11 |
U: AGC CAA TGC ACC AAG AAG |
cDNA |
250+300 |
54-58 |
2 |
MBP |
|
|
|
|
|
|
|
|
|
Metallothionein-like protein |
L: CCG CCT CAG AGG TTA CAT AG |
223 |
|
|
|
|
|
|
|
|
|
|
|
|
|
PRIMERS |
Amplification conditions |
Linkage groupg |
||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01C010 |
U: CGC CCT CTT CTA TTA CTG CT |
cDNA |
160 |
56-58 |
2 |
180 |
56-58 |
2 |
|
|
|
|
|
|
|
Photosystem I |
L: CAC CGC TGC TCT CCT C |
161 |
|
56 |
|
|
58 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01D01 |
U: CCC CGC TAC TGC TAT CTT CT |
cDNA |
155 |
48 |
2 |
155 |
48-58 |
2 |
|
|
|
|
|
|
|
2-cys peroxyredoxin |
L: TGC CAA TCG TGT GCT GA |
155 |
|
|
|
|
|
|
|
|
|
|
|
|
|
PRIMERS |
Amplification conditions |
Linkage groupg |
||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01D04 |
U: TGC ACA CGG CTC TCT GAA CC |
cDNA |
160 |
56-58 |
2 |
160 |
54-58 |
2 |
|
|
|
|
|
|
|
Alpha-tubulin |
L: CGT GCC CAA GGA TGT CAA TG |
161 |
|
56 |
|
|
56 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01D07 |
U: CTG GAA CGG GCA AGA CAG C |
cDNA |
155+300 |
48-58 |
2 |
155+300 |
48-52 |
2 |
|
|
|
|
|
|
|
NeIF-4l7 |
L: GAT GGT AAT CGG GGC GAA AA |
2106 |
|
50 |
|
|
50 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AN01E04 |
U: TGA TGT TAT TGA GGG GTT GA |
cDNA |
1000 |
48-52 |
2 |
250+750 |
48-54 |
2 |
|
|
|
|
|
|
|
Glycin decarboxylase (H subunit) |
L: TCG GTT AGT TTT GTG TTG GT |
252 |
|
52 |
|
|
52 |
|
|
|
|
|
||
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01A03 |
U: CCC ATC TCA CAC ATA ACA AA |
cDNA |
200 |
48-56 |
2 |
MBP |
|
|
|
|
|
|
|
|
|
DAG protein |
L: TCA CTT CCG ACG ACC TT |
200 |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01A06 |
U: GCT GGT CAG GCG GGA TTC |
cDNA |
NA |
|
|
NA |
|
|
|
|
|
|
|
|
|
Polyubiquitin |
L: AAC CCC TTG CCG ATT CCA AT |
1101 |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01C03 |
U: GGC CTC TCC ACC CGC ATT CT |
cDNA |
220 |
54-58 |
1.5 |
220 |
54-58 |
1.5 |
|
|
|
|
|
|
|
Chloroplast ATP synthase gamma subunit |
L: GAC GCC CGT TCA CCA CAG C |
222 |
|
54 |
|
|
54 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01C04 |
U: GTA ACC GGG AGC GTA TTC A |
cDNA |
NA |
|
|
300+800 |
48 |
2.5 |
|
|
|
|
|
|
|
Cytochrome c reductase hinge protein subunit |
L: GCC CAG TTT CAT CTT GTT CA |
155 |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01C10 |
U: GGC CCC CAT CCT AAA ATG AA |
cDNA |
1000 |
48-58 |
2 |
1000 |
54 |
2 |
|
|
|
|
|
|
|
Beta carotene hydroxylase |
L: GAA GGG CCT CCA AAA GCA CT |
21010 |
|
54 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01C07 |
U: ACG CAG AGT AGA AAC CAA CA |
cDNA |
250+300+35 |
54-58 |
1.5 |
300 |
48-56 |
2 |
|
|
|
|
|
|
|
Heat shock protein |
L: TCC CAG ACA AGA CAA ACA AT |
183 |
|
54 |
|
|
54 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01D10 |
U: CCT CTG CTC GCA CCT TGG TC |
cDNA |
250 |
64 |
2 |
MBP |
|
|
|
|
|
|
|
|
|
Thiasole biosynthetic enzyme |
L: GTA GCG CCG GGT CAT CTC C |
250 |
|
|
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01E07 |
U: AAG GGG CAG GGT GGA AT |
cDNA |
350+400 |
54-58 |
2 |
350+400 |
58 |
2 |
|
|
|
|
|
|
|
V-ATPase subunit G |
L: CCT CTG TGC CCG ATA AGA AG |
147 |
|
58 |
|
|
|
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01F10 |
U: CAG GAA AGG GTA AAG ATG G |
cDNA |
150 |
48-56 |
2 |
400+1000 |
48-50 |
1.5 |
|
|
|
|
|
|
|
Cellulose synthase |
L: TGT AGG TGT AGT GGC AGG TA |
1110 |
|
56 |
|
|
48 |
|
|
|
|
|
|
|
|
Amplification conditions |
Linkage groupg |
|||||||||||||
|
primer pairs designed from Pinus pinaster ESTs (d) |
Pinus pinaster |
Picea abies |
Pinus sylvestris |
Pinus pinaster |
Picea abies |
Pinus taeda |
||||||||
|
Gene |
primer sequences (5'->3') |
Expected length |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
Observed length |
T° (range) |
MgCl2 (mM) |
|
|
|
|
AS01F03 |
U: GAG AAC CGA ACA GCA GGA AT |
cDNA |
180 |
56-58 |
2 |
150 |
50-56 |
2 |
|
|
|
|
||
|
|
L: GCA TGA ACT CAG GGG ACC |
176 |
|
56 |
|
|
50 |
|
|
|
|
|
|
|